DEVELOPMENT OF A NOVEL FLUORESCENCE ASSAY BASED ON THE USE OF THE THROMBIN-BINDING APTAMER FOR THE DETECTION OF O6-ALKYLGUANINE-DNA ALKYLTRANSFERASE ACTIVITY

Human O6-alkylguanine-DNA alkyltransferase (hAGT) is a DNA repair protein that reverses the effects of alkylating agents by removing DNA adducts from the O6 position of guanine.Here, we developed a real-time fluorescence hAGT activity assay that is based on the detection of conformational changes of the thrombin-binding aptamer (TBA).The quadruplex

Synaptic plasticity is often explored as a form of unsupervised adaptationin cortical microcircuits to ranchy doodle learn the structure of complex sensoryinputs and thereby improve performance of classification and prediction.The question of whether the specific structure of the input patterns is encoded in the structure of neural networks has bee

To solve the problems of higher energy consumption and lower intelligence of traditional hard hose travelers, a new hard hose traveler with the function of electric drive and self-propelled is developed aveline by modway in this paper.The operational energy consumption of a reel and a polyethylene tube is an important part of hard-hose traveler ene

Glucosamine, monosaccharide from chitosan obtained from the chitin deacetylation process, has been used widely in various fields such as nutrition, pharmacy, and cosmetics.Glucosamine can be obtained from the hydrolysis of chitosan.Enzymatic hydrolysis provides the advantage of mild reaction conditions, environmentally friendly, and high yield.But